How to prevent evaporation during esterification of FAME?

We used the one step method in the lab to obtain FAME for our GC analysis. Many time we see varying degrees of evaporation of solvent (BF3 and internal standard) from our test tube during the 1...

01 February 2020 1,653 3 View

How to reduce static during weighing?

When weighing powder substances, static tend to cause unstable reading on an analytical balance, and most annoyingly, causing sample to stuck and disperse around the mouth of test tube. How do...

01 February 2020 1,817 2 View

How to regenerate a C18 column?

I'm been running Agilent OPA amino acids method but recently my column loss resolution for some of the peaks and will not resolved to baseline. After some discussion with others people in the...

09 October 2019 5,724 4 View

Intensity shifting on amino acids analysis on Agilent 1200 by OPA?

Hi all, I'm currently running amino acids analysis on an Agilent 1200 HPLC system with DAD ( G1315C). I noticed that I've problem reproducing the intensity of my amino acids standard from...

04 May 2019 8,531 4 View

Can anyone help to verify my borate buffer calculation for HPLC?

Hi, I'm trying to make my own borate buffer as per the specification in the picture attached. Concentration of 0.4 N, and a pH value of 10.2. I found a lot of different recipe online and I came...

03 April 2019 5,308 4 View

How to analyze and report GC-FID results (FAME) from data processing software?

Hi, I ran analysis on the concentration of Fatty Acids Methyl Ester (FAME) of freeze-dried fish sample using Shimadzu GC2010Plus and its companion software GC Solution. I first plotted a 4...

01 February 2019 674 4 View

Buffer choices for amino acids by HPLC?

Hi, I'm doing amino acids of animals feed. I used Jajic et al. (2013) 's methods for the acid hydrolysis. Briefly, I will mix 0.5 gram of samples with 7 ml of 6 molars HCl-phenol solution, flush...

08 September 2018 6,404 4 View

Helps with hydrolysis technique for amino acids?

Hi, I need to analyze amount of cysteine spray-coated on animal feed. I read the AOAC (2000) which briefly stated that: (1) Mix 100 mg feed with 5 ml performic acid and oxidized on ice for 16...

07 August 2018 8,703 5 View

Need help in Stock Solution Calculation of Cysteine?

This question is based on the paper by Ogwu & Cohen, 1998. A Simple Colorimetric Method for the Simultaneous Determinat... It was mentioned that a 1.33 mM of cysteine stock solution was prepared...

05 June 2018 1,670 5 View

Colorimetric method for cysteine amino acids?

Hi, I wanted to study cysteine level in animals feed, however, HPLC equipment is not available here. I found this colorimetric method (link at the end of the question) online, but it was...

03 April 2018 7,914 10 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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02 March 2021 3,060 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View