I want to make 4-mer, 6-mer or 10 mer of my recombinant protein. I may like to go even higher multimers as long as it is controlled and defined.
Is there a way to go about it apart from the biotin- stretavidin? Please let me know if anyone has any insight about it.
Thanks in advance
Sudeep
You could use a chemical crosslinker such as BS3. You would probably get a distribution of oligomers, which could be varied by changing the concentration of crosslinker and protein. The crosslinks would occur randomly between surface-exposed Lys side chains and amino termini. The various oligomers could then be separated from each other by gel filtration chromatography or sucrose gradient centrifugation.
Just to set expectations, it certainly won't be 'defined', and even fractionation will not afford a 'pure' population except possibly at the bottom end of the range (4-mer). And as already mentioned above, you will have multiple random contact points and hence different oligomer sizes and shapes (ranging from compact forms to linear strings), which will 'blur' any size separations. Other chemistries could be used but the challenge is the same, and the initial mixture is likely to have broad range of sizes. Yields after fractionation will therefore be modest.
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