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Questions related from Sudeep Kumar
I want to make 4-mer, 6-mer or 10 mer of my recombinant protein. I may like to go even higher multimers as long as it is controlled and defined. Is there a way to go about it apart from the...
14 October 2021 7,627 3 View
I am trying to optimize in vivo delivery of plasmids by non viral methods. I wanted to try out Stearated- Octa-Arginine and stearated-PEG. Please let me know if you know a commercial source where...
13 August 2019 2,327 0 View
Hi All, I need to isolate mouse nasal and/or lung epithelial cells for my experiments. Please share protocols or references regarding this. Thanks in advance, Sudeep
28 March 2019 7,056 3 View
HI all, I am looking for the human derived monocyte/macrophage/DC cell lines that are utilized to demonstrate APC activation capacity of any substance. The markers I want to evaluate are CD80,...
01 March 2019 7,776 4 View
Has anyone have experience with Down-regulation of CD3 and CD4 expression, when stimulating with PMA/Ionomycin and Brefeldin A? Is it due to PMA/Ionomycin or due to the protein transport inhibitor...
29 October 2018 5,325 1 View
Hi all, I am new to the use of lentiviral expression/ gene transfer vectors. From the literature I read, I gathered that lentiviral systems can be used to stably transfect mammalian cells with...
24 September 2018 4,196 2 View
Hi all, I am trying to increase the production of a recombinant protein in 293 FT cell line. I found some papers that suggest that use of valproic acid, which act as HDAC inhibitor and increases...
10 August 2018 2,211 2 View
While going through the haplotype diversity of C57Bl6 and BalbC I noticed that the later has all three haplotypes H2-K, H2-D, and H2-L for MHC-I and both I-A and I-E for MHC-II but C57Bl6 mice...
01 January 1970 4,541 0 View
