Sudeep Kumar

6 Questions 72 Answers 0 Followers

Questions related from Sudeep Kumar

Does anyone has solution for polypeptide/protein multimerization?

I want to make 4-mer, 6-mer or 10 mer of my recombinant protein. I may like to go even higher multimers as long as it is controlled and defined. Is there a way to go about it apart from the...

14 October 2021 7,564 3 View

How to isolate Nasal or lung epithelial cells?

Hi All, I need to isolate mouse nasal and/or lung epithelial cells for my experiments. Please share protocols or references regarding this. Thanks in advance, Sudeep

28 March 2019 6,980 3 View

What are the Human antigen presenting cell lines used for APC maturation marker studies?

HI all, I am looking for the human derived monocyte/macrophage/DC cell lines that are utilized to demonstrate APC activation capacity of any substance. The markers I want to evaluate are CD80,...

01 March 2019 7,737 4 View

How to reconcile with downregulation of CD3, CD4 expression after PMA/Ionomycin stimulation?

Has anyone have experience with Down-regulation of CD3 and CD4 expression, when stimulating with PMA/Ionomycin and Brefeldin A? Is it due to PMA/Ionomycin or due to the protein transport inhibitor...

29 October 2018 5,272 1 View

How to increase recombinant protein expression in 293 FT cells?

Hi all, I am trying to increase the production of a recombinant protein in 293 FT cell line. I found some papers that suggest that use of valproic acid, which act as HDAC inhibitor and increases...

10 August 2018 2,131 2 View

MHC haplotype diversity and its effect in immunity in mice

While going through the haplotype diversity of C57Bl6 and BalbC I noticed that the later has all three haplotypes H2-K, H2-D, and H2-L for MHC-I and both I-A and I-E for MHC-II but C57Bl6 mice...

01 January 1970 4,474 0 View