I would like to ask you about the method for sample storage, extraction, sample preparation and analysis of saccharides and sugar alcohols in needles and leaves of forest trees by LC-MS (or GC-MS). I would need to suppress and inactivate the enzymatic reactions in the samples.
In my opinion, needles and leaves should be immediately frozen in liquid nitrogen after tear off. Then remove water from frozen samples by lyophilization, extract non-structural sugars by ethanol, remove chlorophyl from extracts and then analysis.
Thank you for your answers and help.
Sounds very much like You have a good plan. Might just be that in reality a mix ethanol-water is better for the extraction than pure ethanol. after lyophilisation You will probably want to employ a cutting mill to ensure nice small particles.
For the analysis, try an amine column in HILIC mode using acetonitrile as the weak solvent and water as the strong solvent (gradient from acetonitrile to water).
Since you have them already frozen in liquid nitrogen you can probably skip the lyophilization step. Grind them in a ball mill to get a nice powder. As Kai points out, you'll probably need water in your extraction and definitely need it for your analysis if you use HPLC.
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