We followed the NREL laboratory protocol to analyze reducing sugar after pretreatment of biomass via DNS method. But everytime we found the UV-Abs peak at 370 instead of 540nm.
Suggestions are welcome.
Our team is designing a smart water C&C dashboard for a smart city initiative. As we look into the current smart water system landscape, we couldn't reconcile to a definitive solution that...
16 February 2021 1,094 3 View
I am working with rectal swab samples in the field of colorectal cancer research. Does anyone have experience (or can point towards any relevant literature) of specifically working with proteins...
02 February 2021 3,622 3 View
In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...
01 January 2021 3,271 4 View
Dear all, I converted a PDB file into Mol2 file using Openbabel (H added) and then fixed the bond order number using this command: perl sort_mol2_bonds.pl jz4.mol2 jz4_fix.mol2 When I try to...
22 December 2020 303 4 View
Using targeted degradation of non structural proteins of viral capsid can we lengthen the vaccines effectiveness
21 December 2020 7,200 1 View
Hi all, I am using NAP-5 disposable columns from GE for removing DTT from my protein. Can I use the same column again for the purpose of removing DTT. Can you please mention the protocol for...
10 December 2020 3,199 5 View
I want to reduce the disulfide bridge in my 7kDa protein. My protein has only 1 cysteine at the surface and thus makes a homodimer using a disulfide bridge. I want to reduce the disulfide bridge...
24 November 2020 3,211 5 View
The SAED pattern, we obtained from HRTEM shows the interplanar spacing (d) that corresponds to a higher 2theta value than the Powder crystal X-ray diffraction pattern for the same organic...
02 November 2020 8,073 3 View
Want to understand the scientific mechanisms to know type of soil suitable for agricultural purpose.
22 October 2020 4,941 3 View
I have a liquid that glows when UV light is shined. However, when I use my phone camera I can't see the glow, I just see a violet hue. What is the best way of capturing the fluorescence-glow from...
02 October 2020 8,664 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...
02 March 2021 2,300 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.
01 March 2021 9,101 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...
25 February 2021 7,760 6 View
Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?
25 February 2021 4,075 1 View
Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks
23 February 2021 6,518 3 View
I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.
22 February 2021 4,323 3 View
I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...
14 February 2021 6,049 3 View