Hello everyone,
I am thinking to use mPGK promoter for expressing my gene of interest in human cell lines (first) and later in mouse cell lines. Will it work in human cell line in a similar way like it does in mouse cell line? If using mPGK is not a good strategy then can you please recommend any other weak constitutive promoter which could express in both human and mouse cell lines in almost similar fashion?
I will be thankful for valuable suggestions!