Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

What is the molecular size for crystal violet and reactive orange 16?

I am using methyl violet 10B and reactive orange 16 (RO16) dyes for my Ph.D. research. I'm trying to find the molecular size for both of these dyes. It is possible for me to find these dyes'...

01 February 2021 3,333 4 View

How to find the target displacement to use in displacement control pushover analysis according to ASCE 41-17?

I am performing pushover analysis in Etabs of a steel braced frame but oI do not know what value should I use for target displacement. The height of the building is 20m. I want to know what value...

29 January 2021 2,190 2 View

Can we coat the pellet with graphite spray in place of silver paste for making electrical contact to measure the thermoelectric properties?

I want to coat the pellet because without electrical contact (may be due to the high resistance of the pellet), the V-I plot is not coming in the ZEM-3 instrument. I want to measure electrical...

24 January 2021 836 2 View

What is suitable etching resist coating for polished Titanium Grade 5 surface?

Currently we start a chemical etching project. This project involved etching a polished titanium grade 5 surface part. We facing a problem where by our etch resist coating peel off during etching...

15 January 2021 1,592 3 View

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13 January 2021 2,487 1 View

Variant or strain, what exactly happened to the corona virus ?

The new, more contagious variant of the virus that causes COVID-19 which initially detected in the U.K. and has now been spotted in many of the country. While some have referred to this as a new...

11 January 2021 8,810 5 View

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

09 January 2021 9,320 3 View

The hospitals reported rise in ICU admissions of young COVID patients - Is the new strain of COVID to be blamed?

05 January 2021 3,141 12 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View