Fatin Najiah Mohd Idrus

3 Questions 3 Answers 0 Followers

Questions related from Fatin Najiah Mohd Idrus

What is the recommended time/day to perform transfection in primary human macrophage?

Hi, I am working on silencing TNF-alpha in M1 and M2 primary human macrophage. Most of the literature I found suggesting that the transfection should be performed at Day5 of macrophage...

20 May 2021 8,030 2 View

Can we filter sterilise cytokine such as MCSF for cell culture?

Hi, Does someone has any experience in sterile filtering cytokines such as M-CSF or GM-CSF for cell culture? Is it possible to sterile filter them as I am afraid it will left out in the pore...

31 May 2018 5,790 0 View

Does anyone has any protocol for efferocytosis assay using M2 macrophage and apoptotic bodies from oxLDL?

Hi, I would like to know anyone experienced in conducting efferocytosis study between M2 macrophage and oxLDL (as the apoptotic bodies). Kindly share with me if you have protocol especially...

03 May 2018 5,454 0 View