Does anyone has any experience in measuring thymosin beta 4 protein expression using western blot?

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Jai Ghosh

Just check this reference from PubMed Central:

PLoS One. 2014; 9(10): e106532.

Published online 2014 Oct 1. doi: 10.1371/journal.pone.0106532

Alemu Tekewe Mogus

You need to check the solubility and stability of your proteins in lysis/extraction buffers. Did you check the total cell lysate, soluble fraction and insoluble fractions?

Hend Okasha

Hi, Is ur protein thymosin beta 4 protein found in SDS-PAGE at the same MW compared to std thymosin beta 4 protein? If yes so u have a problem in antibodies used in Western Blot.