Ravinder Reddy Gaddam

15 Questions 12 Answers 0 Followers

Questions related from Ravinder Reddy Gaddam

Does anyone has any experience in measuring thymosin beta 4 protein expression using western blot?

We are trying to measure thymosin beta 4 protein expression in HUVEC cell line and in mouse muscle and brown adipose tissue using western blot technique. However, we are not getting any band or...

08 December 2017 3,942 3 View

Link between inflammation, infection and liver sieve defenestration ?

It is widely known that bacterial infection, inflammation and oxidative stress are the known factors of liver sieve defenestration. However, the mechanisms involve LSECs defenestration associated...

29 August 2016 620 1 View

What is the importance of large gaps in the liver sieve during oxidative stress and inflammation?

Liver Sinusoidal endothelial cells (LSECs) are specialized fenestrated endothelial cells in the liver and contain pores which size range from 50-150 nm. It has been shown that LSECs defenestration...

24 June 2015 513 1 View

What is the best method to measure the ACE activity in liver tissue homogenates?

I am finding difficult to measure the ACE activity in liver tissue homogenates by using the flouroscent substrate. Because of the large amount of other metalloproteases (zinc) present in the liver...

23 June 2015 7,562 5 View

What is the best way to prevent the non-specific hydrolysis of the ACE Fluorescent peptide substrate in pancreas?

I am doing ACE activity assays in pancreas and lungs tissue homogenates by using specific fluoroscent substrate (Mca-RPPGFSAFK(Dnp)-OH) and specific inhibitor (captopril). As compared to lungs, in...

09 September 2014 4,109 0 View

Does anyone know the metabolic products of the substance P through neutral endopeptidase (NEP)?

Substance P undergoes metabolism to produce active biological peptide. Can anyone know the metabolic products peptide sequence of substance P through NEP.

22 July 2014 9,504 2 View

What is the effect of Acute Inflammation on ACE and ACE2

Is there any difference in alteration/activity/expression of the ACE and ACE2 in acute and chronic inflammatory conditions

14 July 2014 3,326 0 View

What is the optimum concentration of captopril in in vitro tissue ACE activity assays?

We are performing the ACE activity assay in pancreatic tissue homogenate by using fluorescent substrate. For that we are using ACE inhibitor captopril but we are not very confident about its...

11 March 2014 1,692 0 View

What is the effect of pH on ACE and ACE2 activity?

Does pH of the buffer show any effect on ACE and ACE2 activity in assays. If it does, what is the optimum pH of buffer for ACE and ACE2 to perform ACE and ACE2 activity assays?

04 March 2014 3,443 4 View

Can we consider Liver fibrosis as an inflammatory disease of the liver?

Is inflammation involved in liver fibrosis? What is the role of renin angiotensin system in liver inflammation and fibrosis? And which inflammatory mediators are activated through RAS in the liver?

29 January 2014 2,227 12 View

What is the advantage of using 5 % milk in TBS-T as a blocking agent over 5 % BSA (Bovine Serum Albumin) in TBS-T

Which is the best blocking agent among BSA and Milk? Does it vary depending on the type of protein?

20 January 2014 1,320 35 View

Is there any relationship between renin-angiotensin system (RAS) and immunity?

If there is, can you provide any references where this relationship is proven?

30 December 2013 9,327 3 View

Can anyone provide the protocol for ACE activity assay in pancreas and liver tissue?

29 December 2013 6,851 0 View

What is the best euthanasia procedure for mice after sepsis and acute pancreatitis without altering or change in any measurable parameters?

29 December 2013 6,793 5 View

What is the difference between the human and mouse Angiotensin II peptide sequence?

RAS system.

19 December 2013 129 3 View