Ravinder Reddy Gaddam

8 Questions 9 Answers 0 Followers

Questions related from Ravinder Reddy Gaddam

Does anyone has any experience in measuring thymosin beta 4 protein expression using western blot?

We are trying to measure thymosin beta 4 protein expression in HUVEC cell line and in mouse muscle and brown adipose tissue using western blot technique. However, we are not getting any band or...

08 December 2017 3,879 3 View

What is the importance of large gaps in the liver sieve during oxidative stress and inflammation?

Liver Sinusoidal endothelial cells (LSECs) are specialized fenestrated endothelial cells in the liver and contain pores which size range from 50-150 nm. It has been shown that LSECs defenestration...

24 June 2015 475 1 View

What is the best method to measure the ACE activity in liver tissue homogenates?

I am finding difficult to measure the ACE activity in liver tissue homogenates by using the flouroscent substrate. Because of the large amount of other metalloproteases (zinc) present in the liver...

23 June 2015 7,524 5 View

Does anyone know the metabolic products of the substance P through neutral endopeptidase (NEP)?

Substance P undergoes metabolism to produce active biological peptide. Can anyone know the metabolic products peptide sequence of substance P through NEP.

22 July 2014 9,449 2 View

Can we consider Liver fibrosis as an inflammatory disease of the liver?

Is inflammation involved in liver fibrosis? What is the role of renin angiotensin system in liver inflammation and fibrosis? And which inflammatory mediators are activated through RAS in the liver?

29 January 2014 2,185 12 View

What is the advantage of using 5 % milk in TBS-T as a blocking agent over 5 % BSA (Bovine Serum Albumin) in TBS-T

Which is the best blocking agent among BSA and Milk? Does it vary depending on the type of protein?

20 January 2014 1,196 35 View

Is there any relationship between renin-angiotensin system (RAS) and immunity?

If there is, can you provide any references where this relationship is proven?

30 December 2013 9,285 3 View

Can anyone provide the protocol for ACE activity assay in pancreas and liver tissue?

29 December 2013 6,809 0 View