Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

What is the best way to prepare anaerobic medium?

Hi, I would like to prepare anaerobic medium for cultivation of gut microflora. I have an anaerobic chamber. How should I remove oxygen from the medium? Is it enough to leave it for 48 hours in...

05 October 2020 3,093 4 View

Any online tools for metagenomics data analysis, specifically 18S rRNA?

Could you please recommend any online tools for metagenomics data analysis for 18S rRNA?

01 March 2020 5,424 5 View

Is it possible to do SEM on a cross-section cut of a fresh leaf?

Hello, I'm working with pulsed electric field applied to lemongrass leaves to generate pores on the cell membrane to enhance essential oil extraction. I would like to know if there is a way to...

20 October 2019 1,364 3 View

How can I preserve leaf samples to measure the trichome density?

Hi. I'm working in a tropical rainforest and I need to measure the density of trichomes, but due the lack of equipment in the station, I need to preserve the samples during 1 month to then count...

10 October 2019 1,460 4 View

Intracellular staining of peritoneal mast cells for cytokine detection by flow cytometry?

Hi! Can anyone suggest a protocol for intracellular staining of peritoneal mast cells for cytokine detection by flow cytometry?

19 March 2019 9,262 3 View

Any experience/opinion on sociolinguistic interviews without the interviewer, i.e., only a questionnaire and the interviewees recording themselves?

I want to interview non-native speakers of English, but I am not a native speaker, so to avoid certain implications of this (unnatural setting, accommodation, etc.), I am thinking of sending them...

11 October 2017 4,867 4 View

Does anyone know of a good reference that describes the biology of monocyte/macrophage recruitment and differentiation during wound healing?

Please send me the title/author if you know of a good reference. Thank you!

04 October 2017 7,695 3 View

How do I do immunocytochemistry staining on non-adherent cells?

Are centrifugations required between every wash step/blocking/antibody incubation in order to not lose the cells? Is there a way to make them adhere through a fixative or some other process in...

03 October 2017 8,611 4 View

Staining plant by Safranine O Fast Green FCF?

14 March 2017 1,404 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...

06 February 2021 2,847 2 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

Which software to use to statistically analyze Telomere Length_TRF_Southern Blot?

I am trying to measure telomere length in an ant species using the TRF method, which is a Southern blot technique. Currently, I am struggling with analyzing the images and would appreciate any...

29 January 2021 604 3 View