I am currently engaged in the analysis of a collection of previously obtained samples, which primarily comprise frozen whole blood. My specific requirement pertains to isolating the plasma component from the aforementioned samples.
However, upon thawing, I have observed a phenomenon wherein the red blood cells (RBC) appear to have undergone lysis. Consequently, I am encountering challenges in effectively separating the plasma from the cellular constituents of the blood. Despite employing the Ficoll-paque gradient method, my endeavors have been met with limited success.
I would greatly appreciate any suggestions or guidance you may have in overcoming this issue.
Thanks!
I think that this is impossible because the fluids are mixed. But if you measure specific components like some plasma proteins or Calcium (this is not present in red cells, if I remember correctly) you may possibly correct the concentrations using the hematocrit value if known or hemoglobin concentration (usually 330 g/l in red cells).
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