Paulina Krzyszczyk

5 Questions 2 Answers 0 Followers

Questions related from Paulina Krzyszczyk

I am confused about the effect of GM-CSF on macrophages, specifically in wound healing...?

I know that, in vitro, GM-CSF is used to polarize monocytes into "M1", before another stimulus, ex. LPS. I also know that chronic wounds tend to suffer from an abundance of M1 macrophages, and...

16 November 2017 4,347 3 View

Does anyone know of a good reference that describes the biology of monocyte/macrophage recruitment and differentiation during wound healing?

Please send me the title/author if you know of a good reference. Thank you!

05 October 2017 7,802 3 View

How do I do immunocytochemistry staining on non-adherent cells?

Are centrifugations required between every wash step/blocking/antibody incubation in order to not lose the cells? Is there a way to make them adhere through a fixative or some other process in...

04 October 2017 8,745 4 View

Do I need to fix cells for immunocytochemistry?

Is it vital to fix cells in all ICC protocols? I am staining for a cell surface receptor, CD163, and plan on imaging immediately after the staining protocol and doing all incubations at room...

22 May 2017 8,533 6 View

Does anyone get a high background fluorescence when imaging cells stained with CellRox Red? Any suggestions on how to reduce?

I am staining primary macrophages in complete media. There is a high background when imaging the media controls as well as the positive control (400uM TBHP) Any suggestion on how to reduce? Thank you!

31 January 2017 6,094 1 View