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Questions related from Paulina Krzyszczyk
Please send me the title/author if you know of a good reference. Thank you!
05 October 2017 7,783 3 View
Are centrifugations required between every wash step/blocking/antibody incubation in order to not lose the cells? Is there a way to make them adhere through a fixative or some other process in...
04 October 2017 8,715 4 View
Is it vital to fix cells in all ICC protocols? I am staining for a cell surface receptor, CD163, and plan on imaging immediately after the staining protocol and doing all incubations at room...
22 May 2017 8,522 6 View
I am staining primary macrophages in complete media. There is a high background when imaging the media controls as well as the positive control (400uM TBHP) Any suggestion on how to reduce? Thank you!
31 January 2017 6,074 1 View