Does anyone can suggest me what concentration of Dispase is better to dissociate human umbilical cord blood MSCs?

25 May 2016 0 5K Report

I need to detach from culture flask human cord blood MSCs in the most gentle manner. Instead of using trypsine, I prefere to use dispase. I read that work concentration vary between 0.6-2.4 U/ml. Does anyone have a suggestion for what value of concentration is preferable for this kind of cells?

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