I have my blood in Paxgene RNA tubes. I'm quantifying with nanodrop and the kit handbook is not clear about the blank to used for nanodrop (to do a dilution in tris-hcl 10mM is what they recommend in the some proportion for the sample and the elution buffer (blank); but the results are similar and I think more correct if I just used the elution buffer to do the blank.)
My ratio 260/230 are about 0,33-0,68 , really low. I want to use it for RNAseq
Best regards,
Thanks in advance,
Mariana