For cytosine methylation there are several methods, the bisulfite method being the most popular but is there anything similar for Me-Adenine?
Mass spec analysis of gDNA digested to nucleosides would be the most reliable.
Single molecule, real-time (SMRT) Analysis software works by measuring the rate of DNA base incorporation during sequencing.
I would appreciate if you answer my DBA research questionnaire. Thank you all in...
10 February 2021 1,990 3 View
Hello, I am currently preparing a preregistered report for experiments in different countries. I am studying motivations/values of students; therefore, I want to conduct several same experiments...
28 January 2021 5,763 3 View
I need to include the bending mode dynamics in my simulation. So far, I am only considering the rigid body dynamics. So I need to implement the following equation: q'' + Kg*q = Phi*F(t), where:...
19 November 2020 4,270 5 View
Dear fellow researchers, we recently conducted an online survey seeking to ask physicians about their perceptions and adoption behavior regarding telemedicine in primary and specialized care. We...
26 October 2020 1,289 6 View
Hi, I am looking for a coenzyme Q10 extraction protocol for fibroblasts, in order to be measured by HPLC. Most articles are very vague explaining their procedure. Thank you very much!
05 October 2020 8,245 2 View
Hi Guys, I would highly appreciate some help/input regarding boolen-stine bootstraping in AMOS. Given that my data violated the multivariate normality assumption I have opted to run bollen-stine...
12 May 2020 2,391 3 View
I am searching for potential project partners (companies) with experience in the carbon coating of metal particles.
03 March 2020 8,208 4 View
I am a master student in Brazil and would like to know if it would be possible to share the Matlab files corresponding to this work (Matlab-code for Traffic Flow Comparison of Roundabouts and...
08 November 2019 4,703 3 View
Hi everyone, we are testing a calibration-free algorithm to estimate the concentration of a unknown material using the LIBS spectra. It is implemented in Matlab and we plan to publish it in...
01 October 2019 3,202 1 View
acidification of soils is a problem for sustainable agricultural use. Liming of soils is a propriate and for a long time used application in agricultur and forest. Therefore new scientific work is...
11 July 2019 10,021 12 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View