17 October 2013 1 2K Report

Is it a Saccharomyces or not? If so, is it a S. pastorianus?

More Chiara Nadai's questions See All
Non-parametric ANCOVA post hoc test?

I am doing a non-parametric ANCOVA (my residuals are not normally distributed and transformation did not help), I have been using the sm.ancova function from the sm package. Now I want to do a...

07 April 2024 8,974 5 View

Both negative and positive clone in my colony PCR?

Hello everyone, I've been trying to clone a bacterial protein from S. aureus into E. coli with pQE30 vector. After ligation and transformation (with Xl1Blue), I screened my colonies (more than...

27 March 2024 1,602 3 View

How to reach the target pH in sterile broth after pre-reduction in the anaerobic cabinet?

Hello, I need your support and suggestions. When I autoclave broth media and put them in my anaerobic cabinet to pre-reduce, the pH will drop of 0.5-1.0 (depending on the medium composition and...

22 February 2024 2,810 1 View

Could you please share any recommendations for works such as books, articles that delve into "work as a masculinity contest" ?

Hi! I am currently working on a research project focused on exploring the dynamics of masculinity within the context of the workplace, specifically delving into the concept of "work as a...

23 January 2024 2,261 3 View

I put in freezer antibodies that must be stored in fridge. Someone can help me?

I bought from Epigentek the EpiQuik DNMT Activity/Inhibition ELISA Easy Kit P-3139-48 and the CA capture antibody must be stored at 4°C but I put in at - 20 °C. I bought from Epigentek the HDAC...

19 December 2023 4,682 2 View

Why SSRs show low recombination fraction in linkage mapping based on SNPs?

Hi, I am building grapevine high-density genetic maps based on SNPs. I have one F1 population and I am using the pseudo-test cross method. I am trying to insert SSRs but, despite the fact that...

14 December 2023 6,245 0 View

Which is the minimum number of images to be taken in immunofluorescence assays?

I need to quantify images from immunofluorescence assays. Which is the minimum number of images to be taken when I take into account the reactivity area of positive cells to a specific marker? If...

30 July 2023 3,417 3 View

Why do I have many mismatched gene after filtering with ESTIMATE package?

Goodmorning, I am trying to use the ESTIMATE package to get immune and stromal score for a database containing microarray mRNA data (Affymetrix) in a cohort of 64 cases. I start with 16158 unique...

24 July 2023 6,390 0 View

Interested in a PhD position in the National PhD Programme in Kinesiology and Sport Sciences (Italy)?

Three-year Ph.D. positions in the National PhD Programme in Kinesiology and Sport Sciences (Italy) for 1 project. Supervisor: Chiara Milanese (Verona, Italy). Ph.D. position for the project...

19 July 2023 8,495 2 View

Which test can I use to compare expression of 1 gene across cancer types?

Good morning, I have downloaded mRNA expression data (RSEM (Batch normalized from Illumina HiSeq_RNASeqV2) (log2(value + 1)) from cbioportal for one gene and I need to compare expression of this...

18 June 2023 7,074 0 View

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How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

08 July 2024 9,201 2 View

Why my yeast colony PCR gel has one specific band , but the size is incorrect even when the plasmid DNA that is originally transformed is correct?

The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...

01 July 2024 2,356 6 View

What are these small structures found when PBMC cells are isolated?

Image attached is at 20X. Cultured media for checking presence of bacteria and yeast and both tests were negative.

27 June 2024 7,473 4 View

Which media will produce more biomass in saccharomyces cerevisiae?

Dear all, For higher biomass for saccharomyces cerevisiae which media is suitable in shake flask? 1. YPD media 2. Basal salt media and what is the optimum C/N ratio ( 5:1 /10:1). Thanks &...

18 June 2024 2,106 0 View

Why no gene deletion in S. cerevisiae using Lox p system?

Lox p was integrated at correct location, but unable to express cre recombinase to delete gene. Performed at different log hour and different Galactose concentration. Even though no gene deletion...

17 June 2024 1,069 3 View

How to normalize fluorescence measurements from different microplate runs?

I'm currently doing an experiment where I'm following the expression of a reporter gene (GFP) along growth using S. cerevisiae. The cultivations last around 48 hours. I observed that the results...

10 June 2024 5,941 3 View

Is it legally permissible to reuse algae biomass following the treatment of non-hazardous laboratory waste?

Hello, community, Could you please clarify whether current legislation permits the reuse of algae biomass after it has been used to treat non-hazardous decontaminated laboratory organic waste?...

05 June 2024 5,040 1 View

Yeast carbon base (YCB) + agar + BSA?

I'm planning to perform BSA degradation using YCB and agar, but I need a protocol. Does anyone have one they'd be willing to share?

02 June 2024 6,885 8 View

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