17 October 2013 1 2K Report

Is it a Saccharomyces or not? If so, is it a S. pastorianus?

More Chiara Nadai's questions See All
How can I calculate the diffusion of water in a sphere (radial diffusion)?

I am measuring the moisture absorption of PET granules by measuring the mass over time. Is it possible to approximate numerically the integrated Fick's law (mass vs time) for the case of the sphere?

22 December 2020 6,359 3 View

Is it possible to fix the intercept in a linear model (lm() in R)?

Dear all, I believe this should be a simple question but after trying and reading I still have no solutions. Basically what I want to do is to run a linear model in R fixing the intercept to a...

26 November 2020 6,897 6 View

Which non-parametric equivalent to the two way ANOVA can be used?

Dear colleagues, I am looking for a non-parametric alternative to the two-way ANOVA as my data are not normally distributed (variances are homogeneous). Friedman test is not a good option as my...

28 September 2020 9,857 11 View

Is it possible to calculate diffusion coefficient through a porous thin film using mass and time?

Hello, I have some porous coating on a hygroscopic substrate. I am measuring the mass change over time of samples left in water. The samples were previously dried. Is it possible to derive from...

09 September 2020 8,818 3 View

Molecular SET-based circuit simulation tools?

Hi! Are there any simulation tool for digital circuits based on Single Electron Transistor (SET) where the dot is single molecule? Is SIMON simulator technology dependent? Thank you

29 May 2020 1,493 3 View

How to properly set k-points in atomistic transport calculations?

Hi I'm interested in analyzing transport in nanodevices. I don't clearly understand how to properly set the number of k-points sampling in transmission spectrum computations. Is it possible, given...

09 March 2020 1,327 4 View

How can I dry an aqueous suspension of polymer nanoparticles?

Can I try to use vacuum oven to obtain a powder that I can then redisperse? Thanks

18 February 2020 3,802 2 View

Does anybody have experience with Igor Pro on macOS 10.15 (Catalina)?

WaveMetrics recommends Igor Pro users to avoid upgrading to Catalina. However, this is only due to the fact that third party XOPs may not run. I therefore wonder if anybody have tried to use Igor...

12 November 2019 6,237 1 View

Why can't I get bands after SDS PAGE?

Hello everyone. I run a SDS PAGE on wheat anthers for my fist time, and I have got this outcome (see annex). In the first line (left to right), I loaded 10ul of ladder ( Spectra Multicolor Broad...

08 October 2019 7,439 8 View

Colour of PVP in water?

I have prepared a solution of PVP (Mw=10k) in distilled water. After sonication for 30 min and 1 day of stirring, the colour of the solution is brownish. Is It correct? Thanks.

05 December 2018 6,804 1 View

Similar questions and discussions
Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

Western blot transfer problem - what could cause it?

Hi everyone, We are doing western blot with yeast protein extracts. We are applying a wet transfer method using Tris-Gly as a transfer buffer (with 10% methanol) and running it with constant...

25 January 2021 4,228 6 View

Can someone explain why my A260/A280 ratio is lower than 1?

We are conducting a measurement of Ribonucleic acid from baker's yeast (S. cerevisiae) (Sigma-Aldrich R6750) concentration using Eon Biotek Microplate reader. We dilute the RNA in various...

24 January 2021 9,830 3 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View

Is MOPS buffer needed for in vitro horizontal gene transfer in thr coculture of two different pathogenic bacterial species?

Actually i am working on possibilities of horizontal gene transfer of resistant gene in broth coculture of two different types of bacteria, and the protocol i follow involves the use of MOPS...

19 January 2021 4,566 2 View

How can I insert antibody sequences into yeast cell to achieve stable and large quantity production?

I have the sequence of the light chain and heavy chain of a certain antibody. I would like to know how to express this antibody in yeast cells to achieve stable and large quantity production. It...

04 January 2021 9,669 2 View

How to prediction non-visible parts of microscopic pictures?

Dear all, I like to ask if someone has experience with evaluating pictures towards the prediction of non-visible parts. I have confocal-laser-scanning microscopic pictures of yeast cells and want...

28 December 2020 4,309 11 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close