I want to order nitrocellulose membrane for western blot and I forgot the dimensions, soft or hard and dimensions?
Dear profile/Dr_Ali_Al-Shawi
Its depend on what you work on
Pls. find the attached files and the references, good luck
Hoping this will be helpful to your project
Regards
Prof. Houda Kawas
http://www.sciencedirect.com/science/article/pii/0003269786902046
http://www.sciencedirect.com/science/article/pii/0165022X8490040X
https://www.thermofisher.com/us/en/home/references/protocols/proteins-expression-isolation-and-analysis/western-blot-protocol/western-blotting-using-nitrocellulose-membranes.html
http://info.gbiosciences.com/blog/bid/203026/pvdf-or-nitrocellulose-which-membrane-is-best
https://www.researchgate.net/post/How_do_you_soak_your_nitrocellulose_membrane_before_transferring_during_a_western_blot
https://blog.citeab.com/western-blotting-nitrocellulose-vs-polyvinylidene-difluoride-pvdf/
http://www.biocompare.com/Bench-Tips/134438-The-Magic-s-In-the-Membrane-Choose-Wisely-for-Best-Western-Blot-Results/
http://www.gelifesciences.com/webapp/wcs/stores/servlet/CategoryDisplay?categoryId=11127&catalogId=10101&productId=&top=Y&storeId=11761&langId=-1
Article Western blotting”: Electrophoretic transfer of proteins from...
21 February 2017 1,526 4 View
01 February 2017 2,481 1 View
01 February 2017 2,353 0 View
01 February 2017 3,704 5 View
The is toxic elements on aquatic organisms
30 January 2017 115 1 View
Trace metals is toxic are they have direct effect on CK
31 December 2016 2,221 0 View
14 February 2014 884 1 View
01 January 1970 1,867 12 View
The genus Ceratium is important species of dinoflagellates and its cosmopolitan distribution in the world oceans and seas also estuaries.Its necessary to know the number of species belonging to...
01 January 1970 1,612 11 View
01 January 1970 6,756 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...
24 February 2021 8,582 1 View
Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...
23 February 2021 8,609 3 View
Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...
23 February 2021 6,266 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View