Dear   profile/Dr_Ali_Al-Shawi

Its depend on what you work on

Pls. find the attached files and the references, good luck

Hoping this will be helpful to your project

Regards

Prof. Houda Kawas

http://www.sciencedirect.com/science/article/pii/0003269786902046

http://www.sciencedirect.com/science/article/pii/0165022X8490040X

https://www.thermofisher.com/us/en/home/references/protocols/proteins-expression-isolation-and-analysis/western-blot-protocol/western-blotting-using-nitrocellulose-membranes.html

http://info.gbiosciences.com/blog/bid/203026/pvdf-or-nitrocellulose-which-membrane-is-best

https://www.researchgate.net/post/How_do_you_soak_your_nitrocellulose_membrane_before_transferring_during_a_western_blot

https://blog.citeab.com/western-blotting-nitrocellulose-vs-polyvinylidene-difluoride-pvdf/

http://www.biocompare.com/Bench-Tips/134438-The-Magic-s-In-the-Membrane-Choose-Wisely-for-Best-Western-Blot-Results/

http://www.gelifesciences.com/webapp/wcs/stores/servlet/CategoryDisplay?categoryId=11127&catalogId=10101&productId=&top=Y&storeId=11761&langId=-1

Article Western blotting”: Electrophoretic transfer of proteins from...