I am interested in looking at the expression of some EMT markers in human breast tumor samples. It's difficult to find good antibodies. Especially in the case of Snail (Snai1), most papers list the company that produced the antibody but not the catalog number. Often times there are several antibodies from one company. Looking to see if anybody tested and identified ones that work well for IHC on FFPE samples. Thank you!
Hi,
I do not have clues about specific antibodies, but did you try to contact the corresponding author reported on the papers? They should tell you the catalogue number, if they used commercially available antibodies. Hope that suggestion may help. Good luck! D.
I completely sympathize! Publishers need to demand authors to specify the catalog numbers. Everest Biotech has a goat peptide antibody shown fit for IHC in mouse: EB07405. However, batch has changed and the confirmation came from the former batch (another thing to worry about!). You should give this one a try and if you get stuck let us know.
Hello,
I would recommend a free antibody database available at labome.com. For antibodies to Snail for IHC you can check the following link: https://www.labome.com/review/gene/human/SNAI1-antibody.html. Santa Cruz Biotechnology has rabbit polyclonal (H-130) sc-28199 ab suitable for IHC on human samples (Liu et al., Mol Oncol. 2015). In addition, Invitrogen has PAS-11923 ab for IHC at 1:200 (Fang et al., Hepatology 2015). Cell Signaling Technology offers C15D3 ab for IHC on human samples at 1:100 (Luo et al., Virchows Arch. 2014).
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