Electrolyte buffer E is for 10ul transfection kit.
Electrolyte buffer E2 is for 100ul transfection kit.
Just wondering if these two can be interchangeable as I have finished the buffer E but still have some E2 buffer left.
Thanks.
Sorry, I can’t say if the buffer details are identical, however you can make up some of the buffers yourself and recycle the tips. One group published their method a couple of years ago: Article A cost-effective approach to microporate mammalian cells wit...
this link is useful
https://www.thermofisher.com/cn/en/home/technical-resources/technical-reference-library/transfection-support-center/electroporation-support/electroporation-support-getting-started.html
regards
Thanks Hassan for the link.
According to Thermo fisher's website:
The E2 buffer has higher osmolarity than E buffer. Higher osmolarity prevents the leakage of electroporation content from the 100 µL Neon® tip, which has a larger hole at the tip end than the 10 µL Neon® tip (pore diameter of the Neon® tips: 100 µL tip = 2.10 mm; 10 µL tip = 0.65 mm).
If my understanding is correct, I could use buffer E2 for 10ul tips, but not buffer E for 100ul tips.
- Badges
- Science method