I was wondering if anybody has tried different PCR cloning kit in the market. As far as I know, there are at least five different kits for cloning blunt-end PCR product.
1. NEB PCR Cloning Kit.
2. Thermo fisher CloneJET PCR Cloning Kit
3. Thermo fisher Zero Blunt TOPO PCR Cloning Kit
4. QIAGEN PCR Cloning Kit
5. Agilent Technologies StrataClone PCR Cloning Kit
I want to know which one is most efficient, with minimal background or maximal cloning fragment size capacity.
What is your recommendation?
Please don't recommend me cloning method that require specific primer design. They are not "PCR cloning".
Thanks.