Can total phosphate (TP) in soil be determined with UV-Vis Spectrophotometer?

Dear All, I would like to know if total phosphate (TP) and total nitrogen (TN) in soil can be determined with UV-Vis spectrophotometer or not. If somebody knows the method, I would like to get...

03 January 2021 7,662 6 View

How to model a fully Binary dataset?

I am having a difficult time modeling a binary dataset that my team has gathered. It is a dataset of different prescriptions per each patient and an outcome of a certain event. The goal is to...

02 February 2020 9,388 4 View

To detect antibiotics by UPLC- MS/MS, how to calculate the targets based on chemical structure to set up method?

Hello, to detect antibiotics residue in wastewater by UPLC- MS/MS, how to calculate the targets based on the chemical structure to set up method. I will detect 3 antibiotics. just need to put...

03 November 2019 9,658 1 View

Are there any published studies using the 20-item Values Inventory since it was first described in 2016?

IF SO, CAN YOU CITE THEM?

07 April 2019 4,062 1 View

Only one peak in heavy fraction is acceptable for qPCR result for DNA-SIP?

Hello, I am doing DNA-SIP for 13C labeling soil samples lately and I checked the SCI papers which have done DNA-SIP work. Generally, after ultracentrifugation, we do density gradient fractionation...

17 March 2019 3,387 2 View

Reliability and validity of an invented survey?

I want to implement a quality improvement protocol, which includes education, on the provision of oral care in an acute care facility. I would like to conduct a pre- and post- survey to the...

16 January 2019 9,250 2 View

Diffusion distance of rice root exudates?

I would like to know the diffusion distance of rice root exudates. How far can rice root exudates affect the microorganisms in bulk soil? I do not mean for soil depth, just horizontal distance. I...

24 November 2018 2,867 5 View

Is it possible to use SSR to investigate the bacterial diversity in soils?

06 August 2017 2,087 4 View

How do you increase metacognition through technology use? Can you give some examples of online or technology-driven program to increase metacognition?

Educators have been encouraging students to increase metacognitive awareness thereby increasing metacognition. With the advantage of technology, developing the metacognitive skills of students is...

08 February 2017 8,243 13 View

Is there any simple way to interpret the following results?

05 December 2015 8,155 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View