Hi,
My aim is to differentiate MSCs from human to cardyomyocyte progenitor cells. The problem is, that after the differentiation the cells do not proliferate any more. Does anybody have a clue why and how to get them growing?
Kinds,
Lothar
what inducer do you use to induce the differentiation?
The differentiation ability of MSCs into cardiomyocyte-like cells is limited by proliferation capacity, and the cell growth arrest does not imply the loss of the differentiation potential of MSCs.
Which passage do you start to do the induction?
For your reference,
Gao Q, Guo M, Jiang X, Hu X, Wang Y, Fan Y. A cocktail method for promoting cardiomyocyte differentiation from bone marrow-derived mesenchymal stem cells. Stem cells international. 2014 Jun 23;2014.
Hi Lothar,
Maybe your cells have differentiated terminally and hence not proliferating. That is the scenario with many terminally differentiated cells. I doubt that they are hardly cardiomyocyte progenitor cells but have differentiated into cardiomyocytes. Now cells are more sensitive to contact inhibition and have a limited tendency to grow. I observed a similar phenomenon in the case of neurons. So if you want to assess their proliferation, you can trypsinize them, culture separately at a large culture surface, maintain them in maintenance media and observe.
Hope it helps!
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line