My topic is innate immunity of mucosal surfaces, I am especially interested in epithelial M cells. Does anybody know a protocol to isolate them?
you can check this links
http://www.jimmunol.org/content/180/12/7840.full
may be useful to you.......
if you have Mabs you can do it by sorting, otherwise laser capture microdissection after staining with lectins Good luck
\
This is how I did it using both in vitro cell culture and isolation from murine Peyer's patches
http://www.ncbi.nlm.nih.gov/pubmed/22385384
GP2 works really well for antibody selection because it's specific for M cells, whereas UEA-1 also binds to goblet cells
PLease check this article............
Microbial Pattern Recognition Receptors Mediate M-Cell Uptake of a Gram-Negative Bacterium
INFECTION AND IMMUNITY, Jan. 2006, p. 625–631 Vol. 74, No. 1
02 March 2021 3,060 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 9,936 2 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View