7 Questions 24 Answers 0 Followers
Questions related from Chintan Bhagat
My system contains 1.8 ml buffer (pH 7.6, Tris sulfate), 1.0 ml 3mM p-NPA (para-nitrophenyl acetate) and 0.2ml enzyme solution. Change in absorbance is recorded at 25 degree.
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I use metaphlan2 for meta-genomic analysis. I am using it on biolinux. I downloaded and put it in my path, but when i type command " python metaphlan2.py metagenome.fastq --mpa_pkl...
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I want to screen some proteins for a human protein using BLASTp. I want to find a unique protein from my query sequences of protein that are non homologous to human proteins. What should be the...
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When I try to revive my clone from -80 degree, it looked as though there was 30-50% viability. After 3-4 hrs of revival, I found that they all are dead. The procedure i follow: 1. Take vial from...
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I have gone through many papers but I have not found a perfect method to do it.
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I am facing problem of cell revival. When I freeze my clone in liquid nitrogen it looks very good. After 10 days when I check in for revival it is not looking good but revival occured. I used...
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