Does anybody know a method for getting rid of hemoglobin present in hemolised sera to test HAI for influenza antibodies?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to develop an academic literacy program for engineering at the higher education level?

Information literacy in higher education integration with curricula engineering

04 August 2024 5,368 3 View

How to Input and Analyze Results for an Asymmetrical Factorial Design in STATISTICA 13.3?

In STATISTICA 13.3, I used the Experimental Design Builder under the Design of Experiments tab to generate a design matrix for an asymmetrical factorial design (1443) with 12 experiments. However,...

29 July 2024 4,155 2 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

What should be the best Lumens range for T8 (120cm) full spectrum LED lamp tubes?

Please (for Arabidopsis), what could be a good Lumens and color range (Kelvin) range for full spectrum LED lamp tubes size T8 (120cm) for each shelve measuring 130x50 cm (length x width) and 60 cm...

11 July 2024 6,078 1 View

What is the relationship between Artificial Intelligence and Communications?

Much is said about AI and the Communication field. Some say that Communications would very much benefit from that intersection. But specifically which areas would benefit from AI? Could it be a...

11 July 2024 5,335 4 View

¿Where to buy the Aspergillus oryzae NSAR1 strain?

Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...

11 July 2024 6,847 0 View

Cross Attention in Transformers: Standard applications of the same ?

What are the standard applications of Cross Attention in Transformer Architectures ?

09 July 2024 9,310 2 View

Time Series Analysis: Has Recurrent Neural Networks (RNN) ever been used on Time Series Analysis ?

Are there standard RNN architectures been applied for Time Series Analysis, forecasting and anomaly detection problems ?

30 June 2024 3,169 8 View

LSTM on Time Series: Has LSTM architectures ever been applied to Time-Series Forecasting ?

Have we ever used LSTM architectures on Time-Series Forecasting and Analysis, and gotten a decent result ?

30 June 2024 6,924 3 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

How to measure accurate concentration of whole protein from RBC?

Although I tried to perform western blotting with protein extract from RBCs, I could not detect the interest of protein due to red background (maybe, heme or hemoglobin ) all over the lane. I...

15 May 2024 6,182 1 View

Where to perform invitro transcription and capping?

I am planning to do invitro transcription and capping for an mRNA vaccine generation. Can anyone suggest whether it should be done on a bench top or inside a laminar hood?

14 May 2024 186 3 View

Is COVID vaccine harmful to cancer or beneficial?

Frankly speaking thrice time affected by COVID although covaxin taken but no harm to cancer patients.it depending upon metastatic position at that time of COVID affected person s . I was patient...

02 May 2024 1,818 3 View

How is the infection of the influenza virus mouse model confirmed?

What is the protocol for infecting the influenza virus mouse model and how is it confirmed?

23 April 2024 434 1 View

Sequences of mRNA vaccines?

For my project I am looking for the mRNA sequences of mRNA vaccines that are currently undergoing clinical trails or that are already in use. I know that the sequences of two Covid-19 vaccines are...

16 April 2024 639 1 View

What are necessary key point one should consider during subunit computational (MEV) vaccine construction?

Clarify a point for Multiepitope vaccine construction that 1) one should construct 3D structure of MEV with protein adjuvant sequence (A.A) or without it. 2)3D structure refinement, validation,...

06 April 2024 9,893 2 View

How design Specific Primers for Reverse Transcription?

Hi :) How should I design primers for (-)ssRNA samples from Influenza A and hRSV viruses? I need to select the protein, locate the gene sequence, and then use it to design primers. Should I...

04 April 2024 4,896 1 View

ELISA kit for measuring serum anti-glycoprotein E antibody concentrations in samples after Shingrix vaccination?

Hello to everyone, I am currently conducting a study on immunogenicity of Shingrix vaccine in hematological patients. After a research in recent publication I noticed that an in house ELISA is...

28 March 2024 4,274 0 View

CD4 population split into CD4hi and CD4lo in the lung ?

I am looking at CD4 T cell populations in B6 mice at 42dpi with influenza B virus. I do perform CD45 IV labeling just before tissue harvest. Now I am running my samples on the 5L Cytek Aurora and...

28 March 2024 1,588 1 View