I am looking at CD4 T cell populations in B6 mice at 42dpi with influenza B virus. I do perform CD45 IV labeling just before tissue harvest. Now I am running my samples on the 5L Cytek Aurora and I see this weird distribution of CD4 staining where it looks like there are two distinct populations of CD4 (see attached image). The CD4hi population (on the far right) is primarily IV protected and the CD4lo population is primarily IV labeled.
I also stain mLN, cLN, pLN and Spleen and they all look normal. This issue appears to be exclusive to the lung
Has anyone else seen this? I have been searching in the literature but haven't found anything helpful.
Thanks!
This CD4hi population is mostly antigen experienced (CD44+). You can also stain for CD44 and CD62L to distinguish naive vs antigen experience CD4hiD44hi CD4T cells.
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