I want to create a conditional KO, specific for Adipose Tissue Macrophages using Cre LoxP system, but I am having trouble how to do it? Any idea???
There seems to be a lot of medicines that are approved for use in Europe and Canada, but not in the US. I understand that there are a variety of reasons for why this might be the case (market...
17 June 2021 8,446 0 View
I want to identify if my transcription factor has enhancer or repressor activity by inhibiting the promoter (whether by introducing mutations on it, or by using inhibitors). To track this...
28 May 2019 8,537 3 View
I have to show electrostatic surface potential of a PDB code. I tried using APBS pymol but I don't understand why it doesn't work. I followed the tutorials available online but did't work. Can...
08 January 2019 8,870 7 View
I have to visualize the interactions (Van der Waals, electrostatic, etc) within a protein. But I don't know how to perform this in discovery research studio. Thank you in advance!
08 January 2019 3,191 5 View
I need to pick one spot of my protein of interest, but in DIGE the same protein from different samples is placed in the same position. How can I separate the samples that are superposed in the...
18 December 2018 7,344 3 View
The proteomics experiment should be designed in an existing transcriptomic experiment model. I will need help in determining the techniques used for my experiment and of course I will accept any...
21 October 2018 1,046 3 View
We are runing 2DE (18cm IEF) strip and try to transfer on western blot on 25V for 30 minute on nitroceklulose but we dont have good result. Do you know which condition is still working this...
10 January 2017 341 2 View
Dear colleges! I am currently working with Gibson cloning, however I am pretty new on this. I would like some recommendations : articles, publications, your personal experience which describes in...
01 January 1970 6,683 3 View
In case you have an example, please use it to underline the difference.
01 January 1970 6,077 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View