I have whole insects in RNAlater but I will only use some body parts for the RNA extraction.
I need to determinate how virulent is a fungus/oomycete strain-specie in comparative with others. I have plants group with and without fungus symptoms. However I found the fungus sources in both...
06 May 2024 8,974 5 View
I have a fluorescent probe that I'd like to test using flow cytometry, so that I can quantify its relative binding to different cell types. Unfortunately, the flow cytometer at my institution does...
02 May 2024 241 3 View
Dear scientists, we are currently conducting a mouse model for wound healing. The process is as follows: make the wound, apply and suture the silicone splint, apply the tegaderm and apply the...
05 April 2024 4,775 1 View
Providers should make an effort to understand local resistance patterns including those of the referring hospitals. Such data are relevant to monitor longitudinal resistance trends and formulate...
18 February 2024 933 1 View
This query is related to the group's assigned journal "Tenets of Specimen Management in Diagnostic Microbiology".
07 February 2024 2,355 1 View
i need to find an existing study with survey questions
20 January 2024 2,570 0 View
I'm a secondary school teacher who is starting a new research in the field of Visual Thinking Strategies (VTS) in Ibi, Spain. We are a group of 4 teachers that have been working VTS in our...
09 January 2024 4,373 1 View
I'm attempting to separate two DNA oligos of length 120bp and 100bp. I've been running them with 2x formamide loading buffer for 3 hours at 65V. I understand constant power is important for the...
28 December 2023 5,456 2 View
Dear all, please comment about this. Thanks Letter: Bacteriophages and Colistin: A Promising Therapy and... Anionic Phage followed by cationic antimicrobial peptide Colistin Sequential Therapy...
23 November 2023 9,444 0 View
For gastroplus modeling - can I determine the D90 from the specified D50 and SD/bins from the particle entry section? I've modeled several scenarios using a specified D50 and SD and # bins, but...
11 September 2023 9,204 1 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,722 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,537 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,618 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 806 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,365 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,827 0 View