Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Is diversity in teaching methods for students important in communicating information?

Is diversity in teaching methods for students important in communicating information?

26 January 2021 3,387 7 View

Which air pollution/air quality books do you recommend for working with environmental engineering students?

I am exploring up-to-date books on air pollution and air quality, and I would like to know which books do you recommend for environmental engineering students.

25 January 2021 3,972 7 View

Why my ladder bands are not properly stained?

Hi. I am doing an agarose gel electrophoresis. After running PCR samples in a 2% agarose gel at 90V x 45' I obtained the results in the image. The ladder bands are not properly stained. For...

14 January 2021 2,316 4 View

How can I model a prediction of a dependent variable using BOTH a predictor at time 1, time 2 AND the change (time 2-1) of this predictor?

I want to model the following: DV: Y at time 3 IVs: X at time 1, X at time 2, change of X from time 1 to time 2 Which is the most appropriate way (preferably in SPSS). I am afraid I cannot use...

10 December 2020 264 3 View

How to explain "Localized Surface Plasmon Resonance" phenomenon of silver nanoparticles in transmission mode of UV/Vis spectroscopy?

The UV/Vis spectrum of my AgNP samples show a global curve at around 450-500 nm, which should be attributed to LSPR of silver, but how could I explain this phenomenon without reflective UV/Vis...

24 November 2020 665 2 View

Can you recommend me tree databases?

Hi. I have slight experience in searching phylogenetic trees, normaly I use TreeBASE or Dryad but some papers that I found doesn't have the resulting trees in these databases or there are genetic...

26 October 2020 582 3 View

Can DNA sequencing help in identification of hybrids amongst butterflies?

Recently I faced a problem while separating 3 species of genus Lethe butterflies (group Woodbrowns -Satyrinae) working along an elevation gradient and different forest habitats in Kedarnath Musk...

15 October 2020 2,916 3 View

Could ANOVAs be used as a substitute for regression models for mediation analysis?

Hello all, I hope you can help me with these questions. I am developing a mediation analysis using the mediation package in R. I need to analyze the influence of perceived learning (Mediating...

18 August 2020 7,521 9 View

Perception on business failure prediction models using the annual report?

27 July 2020 1,141 2 View

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02 March 2021 3,060 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View