I am working on a collagen-made hydrogel and still have a lot of question about the ultrasonication of hydrogel.
Dear Sir,
would you please mention at least one question among these a lot of questions. I guess you have a problems of degradation and gel deterioration when excessive sonication is applied. Regards
Dear Mr Bouaziz,Thank you for your comments (by the way I am a woman). The fact is that I am working on a collagen made hydrogel, and I have problem to make it gelate back after I ultrasonicate it...The other point is that ultrasonication causes air bubbles in my hydrogel, which I would like to avoid because it limits cells growing after reseeding. Thank you very much>
Dear madam,
sorry for being unable to recognize the gender from the name. Well I think the best way to ovoid this problem of bubbles and to improve the gel quality, is to use freeze-thawing cycling instead (or together with) of sonication. Regards
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