What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is there association of SARS-CoV-2 infection rate with meteorological factors?

There are contradictory research papers about the correlation between meteorological parameters and incidence of COVID-19 in some countries worldwide. Is there correlation between the...

15 February 2021 5,806 2 View

Why does the band sizes on Agarose gel electrophoresis differ from the peaks at capillary electrophoresis?

14 February 2021 4,773 4 View

How to calculate the Boiling point for metals at high vapor pressure?

In our experiment, some metal surfaces are exposed to radiation heat under high vapor gas pressure. I would like to ask if there is any way to calculate the boiling point for metals under high...

31 January 2021 249 4 View

What test should I use after a significant factoriual ANOVA?

Dear Colleagues Several resources recommended following significant interaction ANOVA with a simple main effect analysis to break down and interpret the interaction. I am wondering if I can...

29 December 2020 8,316 4 View

In your opinion , What is the percentage of demand for accountants and the percentage of stagnation ?

in your opinion , What is the percentage of demand for accountants and the percentage of stagnation ?

24 December 2020 5,349 3 View

How can increase the follower and citations of my articles?

I am looking for increasing the citations and reading my articles around the world.

13 December 2020 7,074 3 View

Does anyone know how can I display a aperture or point spread function for MIMO array on MATLAB?

Hello, I am trying to optimize an antenna array for MIMO radar. I want to start by using a MATLAB code to display the aperture and the PSF (point spread function) of the array. Could anyone give...

10 November 2020 6,502 1 View

What are the recent research direction of Skin Cancer Detection ?

I am doing a research on Skin Cancer Detection. I have read a lot of redundant research papers about the problems which related to the same topic. Therefore, I wish to know the recent research...

04 October 2020 8,628 1 View

How to do energy optimization of charged molecule in gaussian?

when we want to study a reaction with a charged molecule (+) or (-) located on an atom, how do we optimize its structure with gaussian 09 thanks

29 September 2020 4,873 6 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

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02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View