The cocktail is expensive and we sometimes run out of it. I'm interested in phosphoprotein analysis.
Hi. I use sodium fluoride 10mM as general inhibitor for protein phosphoseryl and phosphothreonyl phosphatases and sodium orthovanadate 0,2 mM that is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases.
Hi valeria,,,,,i would appreciate if you could say how you prepare vanadate solution....
we by the sigma sodium orthovanadate s-6508, that is a dust
and when prepared we store it at -20°C
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