01 January 1970 13 6K Report

Hello,

I'm trying to costain cilia with acetylated-alpha-tubulin antibody (6-11b-1 mouse monoclonal) and something to mark the base of the cilium. My first attempt was with Anti-gamma-Tubulin (AK-15) which I thought would stain centrioles. Unfortunately my first attempt had lots of background staining with nothing that looked convincingly like a centriole. While thinking about ways to optimize my gama-tubulin staining, it occurs to me that somebody might already have a better way to do this.

If somebody could suggest a good antibody for this purpose, I would be very grateful. 

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