Amila Zuko

5 Questions 10 Answers 0 Followers

Questions related from Amila Zuko

How to avoid uneven (intensity) bands during Western Blotting?

I've uneven bands (size, intensity, and staining) when doing Western Blotting and I'm wondering what the problem could be. The protocol: -10% separating gel (0.375M Tris pH8.8; 0.1% SDS; 10%...

15 July 2019 6,727 8 View

What is the difference between the functions of N2 and B27 supplements during ES cell differentiation into neurons?

Several papers base their method of ES cell differentiation into neurons on Bibel et al., 2007 (Nat Prot). When the cells are initially plated for neuronal differentiation the medium contains N2...

23 June 2016 6,725 4 View

How to dissolve PEI (polyethylenimine)?

This concerns linear PEI 25kDa. So far I am aware of two methods: 1: stir the PEI in MQ (1mg/ml) and dissolve by dropping and holding the pH at 2. After its transparant increase pH to 7 -->...

21 April 2016 2,987 22 View

What could be the reason for these strange chromatograms (see attachment) after DNA sequencing?

Lately I'm getting failed DNA sequences and strange sequence peaks in my chromatograms when trying to sequence my plasmid (see attachments). With a previous mini prep sample, the following...

09 February 2016 6,527 4 View

Could someone help me with some Neuronal Tissue Co-IP issues?

I have been doing co-immunoprecipitations for a while now using my own buffers (CHAPS lysis buffer + protease & phosphatase inhibitors) and Dynabeads protein G beads. Useful information: -The...

22 January 2014 5,295 3 View