Jeff Falcone

3 Questions 8 Answers 0 Followers

Questions related from Jeff Falcone

What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

15 July 2020 1,538 4 View

How to purify large amounts of plasmid after restriction digest?

I'm trying to linearize large amounts of plasmid (500 µg - 1000 µg). I can easily digest this amount of plasmid, but when I precipitate with ethanol and resuspend in TE, I'm left with a milky...

04 November 2019 2,787 4 View

Does anybody have a good antibody for staining basal bodies of primary cilia?

Hello, I'm trying to costain cilia with acetylated-alpha-tubulin antibody (6-11b-1 mouse monoclonal) and something to mark the base of the cilium. My first attempt was with Anti-gamma-Tubulin...

01 January 1970 6,449 13 View