Jeff Falcone

6 Questions 11 Answers 0 Followers

Questions related from Jeff Falcone

What is a typical DBC of an affinity column?

I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?

15 July 2020 1,637 4 View

How to purify large amounts of plasmid after restriction digest?

I'm trying to linearize large amounts of plasmid (500 µg - 1000 µg). I can easily digest this amount of plasmid, but when I precipitate with ethanol and resuspend in TE, I'm left with a milky...

04 November 2019 2,875 4 View

Replacement for clonetech DsRed AB that still works for monomeric RFPs

Hello, I am desperately in need of a good mCherry AB. I've been using DsRed rabbit polyclonal from clonetech and it was working excellently - great staining no background. Unfortunately when I...

06 June 2017 4,828 1 View

Neuronal cilia marker?

I'm trying to stain cilia in mouse brains. The only AB that has been successful for me is sc-588 from santa cruz. This also seems to be the only AB that I'm finding used successfully in papers....

06 February 2017 4,343 1 View

Does anyone have an Arl13B antibody that works well for immunofluorescence?

Hi, I'm hunting for good antibodies. I'd love to hear from anyone who has a good arl13b antibody that they are using for immunofluorescence, preferably one that has been used successfully in...

24 January 2017 7,139 3 View

Does anybody have a good antibody for staining basal bodies of primary cilia?

Hello, I'm trying to costain cilia with acetylated-alpha-tubulin antibody (6-11b-1 mouse monoclonal) and something to mark the base of the cilium. My first attempt was with Anti-gamma-Tubulin...

01 January 1970 6,538 13 View