We are doing research with C57BL/6 mice to induce obesity using High-fat diet (D12492-60% kcal% fat).
After 2-week observation, the high-fat diet intake mice have a wavy hair problem and also sample treatment group showed the same. We are curious without any sample treatment mice also having a wavy hair problem.
Please help me out! Is there any problem with the high-fat diet or not?
Reason 1: The dirty cage, mixed up with diet , bedding material makes mice uncomfortable and induces stress.
Reason 2: The diet you are using have high fat makes mouse skin to secrete more oil from body and makes it greasy.
Reason 3: the piloerection, High fat diet, specially the diet you used increases body fat(http://www.epsekishin.co.jp/cn/lsg/service/researchdiets/pdf/NAASO%20poster%202005.pdf)..
increased brown adipocytes acts through UCP1 to regulate thermogenesis. It may nonshivered thermogenesis or thermoneutrality.
the imbalance in protein/carbs/fat makes mice unregulated thermogenesis .
you may check mice body temperature to find it.
good luck for your research.
We faced the same problem (with wistar albino rats) when we used hydrogenated oils or margarine, but when we switched to using natural animal fats as the basis for the diet, this phenomenon disappeared.
That's interesting. I use high fat diet to induce obesity in Sprague dawley rats and haven't encountered such problem. Therefore, this means its strain specific and calls for research.
Reason 1: The dirty cage, mixed up with diet , bedding material makes mice uncomfortable and induces stress.
Reason 2: The diet you are using have high fat makes mouse skin to secrete more oil from body and makes it greasy.
Reason 3: the piloerection, High fat diet, specially the diet you used increases body fat(http://www.epsekishin.co.jp/cn/lsg/service/researchdiets/pdf/NAASO%20poster%202005.pdf)..
increased brown adipocytes acts through UCP1 to regulate thermogenesis. It may nonshivered thermogenesis or thermoneutrality.
the imbalance in protein/carbs/fat makes mice unregulated thermogenesis .
you may check mice body temperature to find it.
good luck for your research.