Premkumar Natraj

3 Questions 2 Answers 0 Followers

Questions related from Premkumar Natraj

Why Does the Molecular Weight of My Protein Differ From the Expected Molecular Weight?

Dear Researchers, I am performing a Western Blot analysis. My protein's expected molecular weight was 96-122 kDa, but I observed it at 60-75 kDa. Is there any problem with my protein preparation...

18 April 2023 6,127 3 View

Does anybody face the wavy hair problem in C57BL/6 mice using High fat diet (60% calorie fat)?

We are doing research with C57BL/6 mice to induce obesity using High-fat diet (D12492-60% kcal% fat). After 2-week observation, the high-fat diet intake mice have a wavy hair problem and also...

11 December 2020 450 4 View

What is the reason for double signal in phosphorylation of ACC (Acetyl-CoA carboxylase) ?

Dear researchers, I am new to Western blot analysis. whenever I am trying to absorb the phosphorylation of ACC (Acetyl-CoA Carboxylase) detecting by Western blot, I always...

28 April 2020 8,875 3 View