Thanks

Thanks

Indeed, it is very difficult to stimulate THP-1 cells to produce ROS. We tried with phorbolester PMA, but without success. With electrochemical methods, theoretically we can measure nanomol concentrations of H2O2, but we could not.

Here are 2 more articles about the cells and H2O2:

Reduced PMA enhances the responsiveness of transfected THP-1 macrophages

to polarizing stimuli

Real-time monitoring of reactive oxygen species production during

differentiation of human monocytic cell lines (THP-1)

Try RAW macrophages instead and confer papers by Amatore, Christian et al.

Best wishes: Monika Bozem

In context of this topic, I would like to bring to your attention a recent review of our group, "Functions of ROS in macrophages and antimicrobial immunity". In this review, we give an introduction to ROS and their sources in macrophages, summarize the versatile roles of ROS in direct and indirect antimicrobial immune defense and provide an overview of commonly used ROS probes, ROS source inhibitors and ROS scavengers (also the difference between ROS scavengers and antioxidants, which are not synonymous, is explained). If you like, please have a look at: Functions of ROS in Macrophages and Antimicrobial Immunity February 2021, Antioxidants 10(2):313 DOI: https://doi.org/10.3390/antiox10020313