Julieta Sepúlveda Angulo

4 Questions 4 Answers 0 Followers

Questions related from Julieta Sepúlveda Angulo

Somebody in México work detecting human cytokines interested in a colaboration in a work related with immunology, parasitism or lipids?

Im interested in made a determination of IL8, IL6 and IL1B production in some samples, but not enought to buy a kit. I´m from Guanajuato, and I´m interested in made a colaboration with other...

06 November 2018 429 1 View

Does any one knows if monocytes THP-1 produce reactive oxygen species, I´m have read about a poor response to LPS, but they can produce them?

I read that they express little CD14, and have a less response to LPS than peropheral monocytes, but I have also read some works that measure the reduction of NBT, ti measure de ROS production,...

31 August 2017 2,305 4 View

How to get reproducible results on NBT reduction assay on Monocytes THP-1 for detect ROS?

I am having problems to have reproducible results in the establishment of my controls for the determination of ROS in THP-1 monocytes by the NBT reduction method, inducing with PMA or LPS. Could...

13 June 2017 2,525 3 View

Does anybody have try an other isolation protocol without using the solvent E; to isolate LPG for Trichomonas vaginalis?

Im traying to isolate te compound by a Extraction with a diferent proportions of Chloroform , methanol and wather, and then with solvent E, but then I have to dry it to evaporate the piridine from...

19 January 2017 4,833 0 View