Histological scoring of acute inflammation in Trachea?

Hello, Does anyone have a detailed protocol for H&E histological scoring of acute inflammation in particularly "trachea"? Thank you. Nazli

05 July 2020 7,932 3 View

How can I import 3ddose file which is out put of dosxyznrc to CERR for plotting DVH?

Hi everyone I have some 3ddose files which are the out put of simulation by dosxyznrc and now I am going to draw DVH in CERR. These 3ddose files are read in Matlab and by using CERR ,they will...

30 January 2020 1,514 4 View

How can the " MAXSTACK" or "STACK size " be changed in egsnrc code?

I had faced with an Error ( stack overflow in $SELECT-PHOTON-MFP) while the dosxyznrc was running. I have figured out the Error related to the number of photon splitting and for removing it , the...

11 December 2019 9,458 3 View

How can I attach an amine group (-NH2) to a carboxyl group (-COOH)?

20 September 2019 5,256 5 View

Has anybody used pronase digestion on the cell culture media? if yes, could you please provide me a brief protocol?

has anybody used pronase digestion on the cell culture media? if yes, could you please provide me a brief protocol?

01 November 2017 4,857 1 View

Any advice on how to find type of regulatory interactions between two genes by using any bioinformatics tool or program?

I have list of pair-wise interactions and I would like to find out the type of interaction (gene X inhibits transcription of gene Y or gene A activates transcription of gene B) between them. Is...

31 October 2017 236 2 View

How can you explain the difference between these data ?

Dear researchers, Here comes a puzzling question. I am having difficulty in explaining a difference in the data of two subcontractors in a scientific way. as we all know, polymers properties are...

23 May 2017 3,384 6 View

Can we plot creep master curve of medical devices by using DMA machine ?

16 March 2017 5,665 10 View

Does anyone have the Arrhenius plot of stress vs. time to rupture of HDPE at room temperature ?

Hello researchers, I am looking for the stress vs. time to rupture curve of HDPE @23C in air, however needs be plotted with Arrhenius method and not TTS (time temperature superposition), any help...

06 March 2017 5,069 3 View

Does anyone know why the negative value obtained for figure of merits?

26 June 2016 3,281 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Why the cells die after stable transfection?

I tried stable transfection in the U87 cell line with the sgRNA-Cas9 All-in-One lentivector. Cells were cultured in a 6-well plate, 4ug plasmid was transfected after 24hs when the cell confluence...

22 February 2021 2,864 4 View