Can you suggest literature studying the conditions information and evidence can influence manager decisions in social impact sectors?

I am working on improving the use of digital innovations at scale globally. Most of the innovations I work with provide relevant knowledge on improving the performance of projects, programs, and...

16 January 2021 1,120 5 View

Can you advise reference publications/standards on characterizing plant genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...

31 December 2020 5,871 4 View

Can you suggest Standard vocabulary for characterizing research and development initiatives (project,program,policy,networks) and their performance?

I am doing text mining on the performance on research for development interventions in low income countries. I need some standard dictionaries and typologies to map the content of the paper in a...

31 December 2020 5,329 4 View

Can you advise reference publications/standards on characterizing animal genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of animal genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of...

31 December 2020 4,924 3 View

How can I construct a matched dataset from CEM function in R?

I could not construct a matched dataset after coarsened exact matching by using CEM package in R. Is there any way to construct a matched dataset like "match.data function in MatchIt package"?

05 December 2020 6,886 3 View

What do you think of Buspiron's effect on OCD?

A study performed at the University of Michigan examined whether brain activity is associated with treatment response to exposure-based CBT. Eighty-seven patients with OCD were randomly assigned...

30 October 2020 1,428 3 View

Can you suggest topic of Mathematics for M.Sc. Thesis in Applied Mathematics ?

01 October 2020 5,677 12 View

Alternative data visitation softwares for Mac?

I am looking for free data insulation software for a Mac instead of Excel and Tableau. Any advice would be appreciated.

20 May 2020 7,317 4 View

Tips and tricks to improve PL signal of a low-yield sample in FTIR spectroscopy?

Hi, I am working on donor spectroscopy in Si. Due to low donor concentration around 10^16 cm-3, the PL signal I get by exciting 670 nm pump is very low, below noise floor. The system is locked-in...

25 March 2020 3,607 4 View

Can I continue ARIMA model despite my time series has heteroscedasticity ?

04 August 2019 3,314 6 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Help with culturing monolayer of human brain microvascular endothelial cells (hBMEC)?

I've been having a hard time forming an endothelial monolayer with good tight junctions. The person who trained me on the culture of endothelial cells worked mostly with mouse BMEC, and he used...

22 February 2021 8,253 1 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View