I'm attempting to clean an FPLC with 1M NaOH to remove protein residue that has built up. I normally just flush the machine with filtered and degassed 20% EtOH and H2O. The last time I tried to filter NaOH in a .22 micron filter, it ate through the filter membrane. My biggest worry with not filtering the solution is that it may clog the FPLC, since there are some particles in the solution from the DI water.
Is there a different method I can use to filter the solution, or should I just leave it as is and run the FPLC?
Dear Mimi:
The 1M NaOH should be filtered to be used in FPLC and usually 0.22 um filters works fine. Filter the solution and then use it. In fact, all the buffers and solutions must be filtered before to be used in this kind of chromatographic system.
Good luck!
You should use a membrane type that is compatible with NaOH. You can find chemical compatibility charts for different types of filter membranes online. Remember to consider not just the membrane material, but also the housing and receptacle material.
I agree with above answers. Check this link
http://m.restek.com/pdfs/GNTS2122-UNV.pdf
Good luck
Ahh, I see now. I was using the wrong filter (Cellulose is clearly not compatible). Thank you all so much!
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