Pros and cons of keto diet?

health professionals have different opinions on keto diet. Some highly encourage it others suggest to avoid. Please provide your opinion or research with reference thank you

04 January 2021 8,056 10 View

Inverted peaks in the Imagelab lane profile?

Hello everyone, I have TIFF images of western blots that were acquired using Syngene chemidoc machine. I have converted TIFF files into 8-bit images using ImageJ (as there was a error opening the...

14 September 2020 4,881 5 View

How toxic is thawed glycerol to cell cultures?

As the title suggests, I would like to know if cells kept in room temperature glycerol for >1 hour are at risk of degradation. I usually have numerous samples that I culture from glycerol...

17 November 2019 4,410 3 View

Problems with 3T3-L1 Adipocytes?

Hi there, I am new working with 3T3-L1 Adipocytes I am facing some issues with these cells. Can anyone suggest me how to overcome this? I purchased 3T3-L1 cells from ATCC and cultured these cells...

04 December 2018 475 10 View

Why Hall Coefficient vary from positive to negative randomly for the same sample at same condition ?

We use a HMS 3000 Version 3.51.5 machine for Hall Effect measurement in our lab. Why does it show positive and negative Hall Coefficient randomly in different for the same sample at the same...

03 October 2017 1,455 7 View

What gauge of wire is suitable for connecting a ground bus on an air table to the signal ground at the back of the amplifier?

I am trying to reduce noise in a single-channel recording experiment. I currently have 1 pA noise. I am thinking to use a heavier gauge wire to connect the ground bus (a copper bar) in the air...

28 September 2016 1,793 4 View

How to arrest Metaphase cells in culture?

I wonder if any one can help me with protocol for Chromosome preparation to analyse through Flowcytometry for plant cells

29 May 2016 4,923 3 View

How can I do a chi squared in SAS that accounts for unequal sample sizes?

21 March 2016 1,714 3 View

Why did Microsatellite amplification succeed in some samples of the same species and failed in others?

Hello every body I am working in a project involving screening of spiny lobster using 100 microsatellite groped into multiplexes.. During my research, I faced some markers that succeed in some...

22 April 2015 3,090 12 View

What is the best way to go around in solving a structural problem using the explicit method on ANSYS?

I am using ANSYS APDL to conduct a structural analysis on a structure under an axial compressive load to be able to determine its ultimate collapse strength and failure mode and I want to use an...

24 June 2014 6,711 6 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View