Or can I assume that starting with the same number of PBMCs in all the wells, I will end up with the same number of macrophages in all the wells? (similar enough to not bother with scraping)
Hey,
It depends on what you really need to know from your test.
Be that as it may, if you have a monocyte-enriched PBMC fraction and seed the same number of cells per plate, you should end up with similar numbers of macrophages (if the differentiation conditions are also the same). In this case, scraping is likely avoidable, even because detaching macrophages without damage is not so trivial.
Cheers
Hey,
In my experience, the detachment of macrophages induce some damage on cells and for that I think it is not a good idea to detach, count and plate them again. If you have a monocyte-enriched PBMC fraction (CD14+), you can assume that at the end you will have similar numbers of macrophages. You can also normalize the ELISA concentration values, for the total protein concentration.
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