Do you put the LSRII machine on standby when switching the tubes, or can you leave it on run at low speed?
Dear Alice,
You need not to do anything else while switching the tubes on LSRII instrument. Just take out your tube by sliding the tube support arm, then place the next tube. no extra steps is to be followed.
Hope this is helpful
Dear Alice,
You need not to do anything else while switching the tubes on LSRII instrument. Just take out your tube by sliding the tube support arm, then place the next tube. no extra steps is to be followed.
Hope this is helpful
Dear Alice,
You need not to do anything else while switching the tubes on LSRII instrument. Just take out your tube by sliding the tube support arm, then place the next tube. no extra steps is to be followed.
Hope this is helpful
On any LSR/Fortessa/X20 analyzer, which rely on the operator for fluidics management, it is best to exploit the time between samples to clean the flow cell and the SIP, so that no events from the previous sample are carried on and be acquired during the next one. So, from a practical point of view, placing ddH2O tube (filled about to 2/3-half volume) and shifting to high speed setting (on acquire) while preparing the next sample is a good idea. Remember to go back to your chosen acquisition speed before placing the new sample tube. Keep in mind that the ddH2O will slowly be depleted by the cleaning procedure, especially if you leave the tube support arm open for longer times (which does not result in water -or sample - flowing through the flow cell, but directly to the waste). Never leave the instrument with no tube (or an empty tube) on the SIP on "acquire" for more than a couple seconds (which is the time you should employ to change tubes). When leaving the instrument on stand-by, always place a ddH2O tube (filled to about half or less) on the SIP.
If you are acquiring very "dirty" samples (lots of dead or "sticky" cells, debris, very large cells, clumps, etc...), which have a higher risk of clogging the instrument, switching to a cleaning solution tube (diluted bleach) and then ddH2O between samples would be ideal. Obviously, in these cases, filtering your samples with a cell strainer would be good practice as well..
- Badges
- Science method