Alice Chan

5 Questions 3 Answers 0 Followers

Questions related from Alice Chan

Why does the water-soluble cholesterol crystallize in medium?

I am treating cells with water-soluble cholesterol. (https://www.sigmaaldrich.com/SG/en/product/sigma/c4951) Most papers recommend 1-10mM. Upon treatment of cells in medium, even within 10...

13 January 2022 2,416 3 View

Do you leave flow cytometry machine on run or standby while switching samples?

Do you put the LSRII machine on standby when switching the tubes, or can you leave it on run at low speed?

24 June 2020 3,188 4 View

Which buffer should I use for cell viability and cell cycle flow cytometry analysis of lymphocytes?

I want to measure cell cycle after a chemotherapy treatment of a B lymphocyte cell line. It may cause cell death. According to https://www.rockefeller.edu/fcrc/cellsorting/, I should use DPBS...

10 June 2020 8,246 4 View

How do you properly set unstained and single stained controls for flow cytometry experiments?

I want to compare the cell cycle profile of an untreated sample to a chemotherapy-treated sample using several standard flow cytometry methods: - PI + Click-it Edu + pHH3 (fixed) - PI + Annexin V...

10 June 2020 6,310 3 View

Where can I find RNA seq data of knockout cell lines?

I want to observe differential gene expression of cells that have a knockout of this gene versus another type of cell that normally does not express this gene. Do you know where I can find the...

11 November 2019 6,108 1 View