I used Immobilion transfer membranes which allow to transfer protein higher than 20000, my complex is 42000
I would like to ask you a question concerning meanwhile / temporary uses for vacant buildings (or areas) reactivation and their application in respect to the regulatory framework. What is...
28 July 2020 9,239 9 View
Hello, I read the paper via IEEE. I would like to compute the K coefficients using Excel Solver but honestly I have not understood how to do it. Could you explain me how to do it? Do you have an...
05 July 2020 2,855 1 View
Hello. I am new to the GSH assay and I am learning about it online. I performed it this week for the first time. My protocol said to add DTNB and GR to my GSH standards + samples - allow 30 sec...
24 January 2019 8,328 1 View
Trying to measure the GSH:GSSG ratio in my ES cells. I found several protocols for both GSH and GSSG measure, but mostly from blood or tissue. Not sure what to choose for my purpose. Does someone...
25 November 2018 8,542 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...
06 August 2024 1,752 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
what is the best research evidence for psychological interventions for Bipolar?
01 August 2024 6,023 2 View