Trying to measure the GSH:GSSG ratio in my ES cells.
I found several protocols for both GSH and GSSG measure, but mostly from blood or tissue. Not sure what to choose for my purpose.
Does someone has one from cell lysate?
Hi,
Protocol that works on tissue often works on cell lysate. Try to adjust protein concentration of your lysate, to that of the tissue homogenate, as described.
sincerely
marc Conti
Take a look at the reference below. It describes the protocol for enzymatic recycling measurement of total GSH and GSSG levels from different sources, including cells in culture. We use this protocol frequently, but for animal tissues.
Irfan Rahman, Aruna Kode & Saibal K Biswas. 2006 .Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. Nature Protocols 1: 3159–3165. 10.1038/nprot.2006.378
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